ࡱ> sur bjbj .Tyys* 66666JJJ8DJU\b $Tx,8U:U:U:U:U:U:U$Y[n^U6^U664sU668U8UJdO[oJ>`M&$UU0UMB5\5\LOO,5\6P0^U^UjU5\ : Information for Selected RPPA Project Please change the file name to include PI name and date and email the filled form to core director Please scroll down for iLab registration information PI name__________________________________________ Contact name____________________________________ Project Title_____________________________________ Project description or brief abstract: Species: Human ___ Mouse ______ Other (specify)____________ Sample Type: Tissue___ Cell Line_____ Other (specify)____________ Estimate date of sample delivery ________________ iLab project registration date (see next page for details)_____________ Please fill the form below: Group #Sample Group Name or Treatment ConditionBiological Replicates*1234Total Please expand the table as needed. *Recommended sample replicates: Sample size (number of samples per experimental group) for RPPA project depends on the project goal, sample types, and previous data/knowledge. The exact number of samples for the project should be discussed among the investigator, core director, and statistician during the project initiation phase. As a starting point, we recommend the following biological replicates for each sample. The Core routinely spots all protein lysates in triplicates of arrayed slides. These are technical replicates. Sample TypesBackground# of biological replicates Cell lines or cell cultures>=4Mouse tissues/body fluidPure background>=5Mouse tissues/Body FluidMixed background>=8Human samples >=10 (consult statistician)*The reason that 4 replicates are recommended for cell lines is that 3 replicates are absolutely necessary to generate statistically significant data, and the 1 extra allows room for error or outlier, thus increase the possibility of obtaining statistically significant data. Sample submission important notice: All LYSATES samples (when your own lab extracts protein) should be stored in the same type 1.5ml-microcentrifuge tubes with tight cap (i.e. Fisherbrand, catalog number 02-681-320). Please only place labels on TOP of the tube, NOT on side of the tube. Only HAND WRITE on side of the tube. If your lab would like to ask our core to perform tissue lysate extraction, ALL TISSUE samples should be stored in specified tubes (Qiagen, Cat#990381) which are used for our TissueLyzer instrument. Sample shipping addressMichael Nguyen/Shixia HuangAlkek Building for Biomedical Research R551COne Baylor Plaza, MS:BCM130Houston, TX 77030Tel: (713)798-8935 E-mail: shixiah@bcm.edu  Shixia Huang, Ph.D. Director, Proteomics Core Dan L. Duncan Cancer Center Associate Professor, Dept. Molecular & Cellular Biology Alkek Building forBiomedicalResearch, R507 One Baylor Plaza, MS130 Houston, TX 77030 713-798-8722 (Office) 713-798-8935/6740 (lab) 713-798-1370 (Fax) E-mail:  HYPERLINK "mailto:shixiah@bcm.edu" shixiah@bcm.edu Welcome to iLab Solutions at ! BCM is excited to launch a web-based tool to help manage central resources at the institute. We will be rolling out the software in several phases. The Antibody-Based Proteomics Core would like to invite you to register for an iLab account so you can access core services through the new on-line system. Please follow the instructions below to complete the one-time registration process and get started working with the core! If you already have an account with iLab, you do not need to register again. Simply login and click on core facilities link in the left menu to find the new core listed. BCM Users: Register with iLab  HYPERLINK "https://bcm.ilab.agilent.com/account/login" Click here to login and register:  HYPERLINK "https://bcm.ilab.agilent.com/account/login" https://bcm.ilab.agilent.com/account/login (click BCM user link once on page). Login using your BCM username and password on the BCM single sign on page. Upon successful authentication, you will redirect into the iLab system. Select your lab from the pull-down list, provide your phone number. Submit the completed registration form. Submission will notify your PI of your lab membership request. You will be sent an email with basic instructions on using the system at this time. However, you will not be able to place orders with your new account until your PI has approved your lab membership request. Non-BCM Users: Sign up for iLab Account Complete the account sign-up form here: HYPERLINK "https://bcm.corefacilities.org/account/signup/43?sc_id=3060"    HYPERLINK "https://bcm.ilab.agilent.com/account/43/signup?sc_id=3060" https://bcm.ilab.agilent.com/account/43/signup?sc_id=3060 You will receive an email with account information and basic instructions to get started using the system once your account has been approved. You can also access the core's webpage directly, which has login and registration links for all users in the upper right corner.  HYPERLINK "https://bcm.ilab.agilent.com/service_center/show_external/3060/BCM_Proteomics" https://bcm.ilab.agilent.com/service_center/show_external/3060/BCM_Proteomics If you have any questions, concerns, comments or suggestions, please use the 'leave iLab feedback' link in the upper right hand corner once you are logged in. Alternatively, you can email us at  HYPERLINK "mailto:support@ilabsolutions.com" support@ilabsolutions.com. 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